In Vitro Culture and Characterization of a Mammary Epithelial Cell Line from Chinese Holstein Dairy Cow

In Vitro Culture and Characterization of a Mammary Epithelial Cell Line from Chinese Holstein Dairy Cow

BACKGROUND The objective of this study was to establish a culture system and elucidate the unique characteristics of a bovine mammary epithelial cell line in vitro. METHODOLOGY Mammary tissue from a three year old lactating dairy cow (ca. 100 d relative to parturition) was used as a source of the epithelial cell line, which was cultured in collagen-coated tissue culture dishes. Fibroblasts an...

IN VITRO MAMMARY GLAND MODEL: EStABLIShMENt AND ChARACtERIzAtION Of A CApRINE MAMMARY EpIthELIAL CELL LINE

Isolation and characterization of a spontaneously transformed malignant mouse mammary epithelial cell line in culture.

A method is described that permits the selection of spontaneously transformed mammary epithelial colonies from an untransformed mouse mammary epithelial cell line, NMuMG, and utilizes a long-term anchorage-independent growth of the transformants on soft agarose. These transformed cells (NMuMG-ST) are shown to be distinguishable from the untransformed cells by morphology, growth characteristics,...

Establishment of Mammary Gland Model In Vitro: Culture and Evaluation of a Yak Mammary Epithelial Cell Line

This study aimed to establish yak mammary epithelial cells (YMECs) for an in vitro model of yak mammary gland biology. The primary culture of YMECs was obtained from mammary gland tissues of lactating yak and then characterized using immunocytochemistry, RT-PCR, and western blot analysis. Whether foreign genes could be transfected into the YMECs were examined by transfecting the EGFP gene into ...

effects of aflatoxin b1 on csn3 gene expression under in vitro cultured iranian holstein cow mammary epithelial cells

aflatoxins are toxic compounds which are produced by aspergillus flavus and a. parasiticus. these toxins can be transmitted into the animal body via contaminated feed. when these toxins enter the animal body, they can penetrate into different tissues. one of the most important tissues which can be influenced by aflatoxin is the mammary gland. milk protein is one the most important factors in de...